- Presented at: Drug Discovery & Development 2022
- Sponsored by: LUMINEX A DiaSorin Company
- Hans-Dieter Zucht, PhD
Chief Technical Officer, Oncimmune Germany GmbH
- Discuss autoantibodies and how they are relevant immunological biomarkers.
- Explain how autoantibodies can also target cytokines, receptors they are involved in immune regulation.
- Explain how autoantibody multiplexing and individual assays can be deployed during clinical research.
Cancer still accounts for nearly 1 in every 4 deaths worldwide. New immunotherapies have the power to induce durable responses in patients with fatal cancers, but only a small percentage of patients are responsive. Not only that but clinicians are also faced with new challenges with managing harmful immune-related adverse events (irAEs). A largely overlooked immune cell type in the context of immunotherapies are B cells, which can exert both anti-tumour and tumour-promoting effects by providing co-stimulatory signals and inhibitory signals for T cell activation, cytokines, and antibodies. B-cells produce anti-tumour antibodies, which can potentially mediate antibody-dependent cellular cytotoxicity (ADCC). It is well established that many cancer types induce an antibody response, which can even be used for early diagnostic purposes. Besides anti-tumour antigen (TAA) antibodies, cancer patients also produce autoantibodies that bind to self-antigens. Breakthrough of tolerance and elevated levels of autoantibodies is also a prominent feature of many autoimmune diseases. Hence, it is particularly relevant to investigate irAEs resembling autoimmune phenotypes.
In this presentation, we will discuss how autoantibody (AAB) profiling using the bead-based multiplex Luminex® Technology combined with machine-learning procedures offers a unique tool for biomarker research. The highly dimensional data can be used to extract predictive models, identify biomarkers, create patient subgroups and also characterize regulatory networks involving cytokine and receptors suggesting that autoantibodies actively participate in immune regulation. Case studies for applications in melanoma CPI treatment and other cancers will be discussed.